11191-100ML Display Image

Histopaque-1119, sterile-filtered, density: 1.119 g/mL

Code: 11191-100ML D2-231

Application

Histopaque®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and gran...


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Your Price
£108.00 100ML

Application

Histopaque®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood.

General description

Histopaque®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a density of 1.119 g/mL and pH 8.8–9.0.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Principle

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

application(s)hematologyhistology
density1.119 g/mL
impuritiesendotoxin, tested
Quality Level200, 400
shelf lifeExpiry date on the label.
sterilitysterile-filtered
storage temp.2-8°C
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11191-6X100ML
Unit:EACH
List Price: £494.00
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