Application

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Linkage

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 5, 10, 25 g in glass bottle