The 3M™ Allergen Protein ELISA Kits are intended for the detection of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

The 3M™ Allergen Protein ELISA Kits utilize a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

• Designed to detect both processed and unprocessed target allergen proteins
• Test can accommodate environmental swab, CIP rinse water and food samples
• A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time
• 3M™ Environmental Collection Swabs sold separately