B2904-100MG Display Image

Bluo-Gal, reagent for selection of recombinant bacterial clones

Code: B2904-100MG D2-231

Application

Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyr...


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Your Price
£126.00 100MG

Application

Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Packaging

100, 500 mg in poly bottle

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

assay≥98% (HPLC)
formpowder
gradefor molecular biology
InChI keyLINMATFDVHBYOS-MBJXGIAVSA-N
InChI1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
Quality Level200
SMILES stringOC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
solubilityDMF: soluble
sterilitynon-sterile
storage temp.−20°C
suitabilitysuitable for substrate for β-galactosidase
Code
Description
Unit Size
List Price
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B2904-500MG
Unit:500MG
List Price: £431.00
Source:List Price
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Cas Number97753-82-7
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