B4287-25G Display Image

Bovine Serum Albumin, BioReagent, for molecular biology

Code: B4287-25G D2-231

Application

Select the right albumin for your application with the Albumin Selection Guide

A non-acetylated protein suitable as a blocking agent in Southern blotting. I...


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Your Price
£188.00 25G

Application

Select the right albumin for your application with the Albumin Selection Guide

A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

Protease free Suitable for blocking non-specific protein binding sites in hybridizations Heat shock fractionated

Packaging

1, 5, 25 g in poly bottle

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

assay≥98%
biological sourcebovine
foreign activityProtease free
formlyophilized powder
Gene Informationbovine ... ALB(280717)
gradefor molecular biology
mol wt~66 kDa
originUSA origin
packagingpoly bottle of
pH7
product lineBioReagent
purified byheat shock fractionation
Quality Level200
solubilitywater: soluble (40 mg/ml)
storage temp.2-8°C
technique(s)western blot: suitable, ELISA: suitable, electrophoresis: suitable
UniProt accession no.P02769
Code
Description
Unit Size
List Price
Qty
B4287-1G
Unit:1G
List Price: £21.06
Source:List Price
ADD
B4287-5G
Unit:5G
List Price: £98.40
Source:List Price
ADD
Cas Number9048-46-8
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