C30-100MG Display Image

Catalase from bovine liver, aqueous suspension, 10,000-40,000 units/mg protein

Code: C30-100MG D2-231

Application

Catalase from bovine liver is used for the following applications:Dielectrophoretic field-flow fractionation (DEP-FFF): It is a chromatographic method in which ce...


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£46.70 100MG

Application

Catalase from bovine liver is used for the following applications:Dielectrophoretic field-flow fractionation (DEP-FFF): It is a chromatographic method in which cell elution times reflect the positions of cells in a hydrodynamic flow profile under the control of sedimentation, DEP and hydrodynamic lift forces, FSED, FDEP and FHDL, respectively. The DEP-FFF buffer consists of catalase along with other ingrediants.GC-MS analyses.Deionization.GC-FID analysis.5Functional analysis of blood vessel.

Catalase from bovine liver has been used for measuring the hydrogen peroxide conetent in cancer tissue homogenates. It has also been used to test the effect of organophosphate insecticide chlorpyrifos-ethyl (CE) [0,0-diethyl 0 (3,5,6-trichloro-2-pyridyl) phosphorothioate] on its enzyme activity.

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Biochem/physiol Actions

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Caution

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

General description

Catalase from bovine liver contains 506 residues. It is a tetramer and each monomer corresponds to a molecular weight of 61 kDa. The active site in each momomer comprises nicotinamide adenine dinucleotide phosphate (NADPH) and iron binding region.

Preparation Note

This product is a crystalline suspension in water containing 0.1% thymol with activity of 10,000-40,000 units/mg.

Unit Definition

One unit will decompose 1.0 µmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

biological sourcebovine liver
concentration20-50 mg/mL
formaqueous suspension
Gene Informationcow ... CAT(280743)
isoelectric point5.4
mol wttetramer ~250 kDa
Quality Level200
shipped inwet ice
specific activity10,000-40,000 units/mg protein
storage temp.2-8°C
UniProt accession no.P00432
Code
Description
Unit Size
List Price
Qty
C30-1G
Unit:1G
List Price: £302.00
Source:List Price
ADD
C30-500MG
Unit:500MG
List Price: £164.00
Source:List Price
ADD
Cas Number9001-05-2
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