Application

Analytes: Albumin, β-2-Microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4) Recommended Sample Type: Canine urine Recommended Sample Dilution: 25 µL of 1:500 diluted canine urine for normal subjects; optimal dilutions for disease subjects should be determined Assay Run Time: Primary incubation of 2 hours, followed by a secondary incubation of 1 hour, both at room temperature (20-25°C) Research Category: Toxicology

Components

Canine Kidney Toxicity Panel 2 Standard, 1 vial, lyophilizedCanine Kidney Toxicity Panel 2 Control 1, 1 vial, lyophilizedCanine Kidney Toxicity Panel 2 Control 2, 1 vial, lyophilizedAssay Buffer, 2 bottles, 30 mLWash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mLCanine Kidney Toxicity Panel 2 Detection Antibodies, 1 bottle, 5.5 mLStreptavidin-Phycoerythrin, 1 bottle, 5.5 mLMixing Bottle, 1 bottleSample Plate, 96 well format, 1 plate2 Foil Plate Sealers

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

As one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers. In pharmaceutical and chemical industries, renal function is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species. MILLIPLEX^® Canine Kidney Toxicity Panel 2 is a 4-plex kit to be used for the simultaneous quantification of any combination of the following analytes in canine urine samples: Albumin*, β-2-microglobulin (B2M)*, Trefoil Factor 3 (TFF-3)*, Retinol Binding Protein 4 (RBP4)*. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. *Urinary albumin, B2M, TFF-3 and RBP4 are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Toxicity

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: See kit protocol for individual analyte sensitivities.

Please contact Technical Service for linearity of dilution.

Specificity

Cross ReactivtyApproximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel.