D5758-5ML Display Image

Diethyl pyrocarbonate

Code: D5758-5ML D2-231

Application

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

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Your Price
£44.30 5ML

Application

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

DEPC is a potent nuclease inhibitor.0.1% solution of DEPC is used to inactive RNase. It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles.

General description

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.

Packaging

5, 25, 50, 100 mL in glass bottle

assay96% (NT)
biological sourcesynthetic (microbial fermentation/ cell culture)
bp93-94 °C/18 mmHg (lit.)
density1.101 g/mL at 25 °C (lit.)
formliquid
InChI keyFFYPMLJYZAEMQB-UHFFFAOYSA-N
InChI1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
Quality Level200
refractive indexn20/D 1.398 (lit.)
shipped inwet ice
SMILES stringCCOC(=O)OC(=O)OCC
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
D5758-25ML
Unit:25ML
List Price: £157.00
Source:List Price
ADD
D5758-100ML
Unit:100ML
List Price: £464.00
Source:List Price
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D5758-50ML
Unit:50ML
List Price: £270.00
Source:List Price
ADD
Cas Number1609-47-8
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