FGF-2, also known as basic FGF, is a growth factor that controls fundamental biological processes and is qualified for different applications including industrial cell-based meat production, cell culture and cell therapy manufacturing.

FGF-2-STAB® is a stabilised growth factor that offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes. FGF2-STAB® retains full biological activity even after twenty days at 37°C. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required.

FGF2-STAB has an expected ED50 of less than 0.1 ng/mL, as determined by NIH-3T3 cell proliferation assay. The ED50 value of FGF-2 STAB® is about 5-times lower than that of the wild-type FGF-2, as determined by a NIH/3T3 proliferation assay. Under the same experimental conditions, less FGF-2 STAB® is needed to achieve a given effect on cell proliferation, thanks to the higher stability and prolonged half-life of FGF-2 STAB®. Thanks to this competitive advantage, lower dosage of FGF-2 are required in your cell culture applications (up to 10x).

• FGF-2 is produced in plant seeds of Camelina sativa bred in our laboratories in Strasbourg, France
• ≥95% measured by SDS page, and resolved under reduced (R) and non-reduced (NR) conditions
• The biological activity of recombinant FGF-2 STAB® was determined by its ability to promote the proliferation of 3T3 cells cultured in adherent condition. Cell proliferation was measured using non-radioactive colorimetric assay and monitored by absorbance. ED50 is 0.1 ng/mL
• Low endotoxin level ≤0.005ng/µg
• Xeno-free
• Lyophilized, to store at -20°C to -80°C, stable for up to 12 months when stored at -20°C to -80°C. Once it is reconstituted it can be stored at -20ºC or -80ºC for up to 1 year