Application

Monoclonal ANTI-FLAG^® M2 antibody produced in mouse has been used in: immunoblotting immunoprecipitation immunocytochemistry immunofluorescence ELISA EIA chromatin immunoprecipitation electron microscopy flow cytometry supershift assaysBrowse additional application references in our FLAG^® Literature portal.

General description

Anti Flag M2 antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent. F1804 is an affinity purified, FLAG M2 antibody, increasing sensitivity in most applications.Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Packaging

5 mg in poly bottle

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk

Storage and Stability

Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.