Application

Monoclonal Anti-HLA Class I Antigen-FITC antibody produced in mouse has been used in: flow cytometric analysis immunoprecipitationcomplement mediated cytotoxicityimmunocytochemistry

FITC Monoclonal Anti-Human HLA Class I Antigen may be used for immunochemical analysis of HLA Class I antigens; Studies of HLA-A,B,C expression on embryonic and neoplastic tissues; and/or the purification of HLA-A,B,C antigens.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Human HLA Class I Antigen (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human tonsil leukocyte membrane preparation. Class I proteins are highly polymorphic. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain.

The function of HLA class I molecules is to present antigen to CD8+ lymphocytes. They are involved in the regulation of positive and negative selection of CD8+ T cells by thymic epithelial cells during T cell maturation. These molecules are important in allograft rejection and in the linkage of certain haplotypes in autoimmune diseases. Expression of HLA class I antigens may be modulated in pathological states. Malignant cells may lose these antigens while hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may display enhanced reactivity. Increased expression of these antigens is described in muscle cells affected by muscular dystrophy, inflammatory myopathy and other neuro-muscular disorders.

Immunogen

human tonsil leukocyte membrane preparation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Specificity

Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.4th Workshop: code no. P15th Workshop: code nos. BP166, BP288, BP407, B 005