Application

Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway﹤﹤﹤17>>>.

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Packaging

1000 units in glass bottle

2000, 5000 units in poly bottle

Physical form

Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight

Preparation Note

Prepared from G7877

Unit Definition

One unit will oxidize 1.0 µmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.