K1377-5G Display Image

Kanamycin sulfate from Streptomyces kanamyceticus, powder, BioReagent, suitable for cell culture, suitable for plant cell culture

Code: K1377-5G D2-231

Application

Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.

Biochem/physiol Actions

Mode of Act...


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Your Price
£162.00 5G

Application

Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding. Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Caution

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

General description

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100µg/mL.

Chemical structure: aminoglycoside

Packaging

1, 5, 25 g in poly bottle

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2µm filter.

antibiotic activity spectrummycoplasma, Gram-positive bacteria, Gram-negative bacteria, mycobacteria
application(s)agriculture
biological sourceStreptomyces kanamyceticus
colorwhite to off-white
formpowder
impurities≤5% kanamycin B
InChI keyOOYGSFOGFJDDHP-KMCOLRRFSA-N
InChI1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
mode of actionprotein synthesis | interferes
potency≥750 µg per mg (dry basis)
product lineBioReagent
Quality Level200
SMILES stringOS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
solubilityH2O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
technique(s)cell culture | plant: suitable, cell culture | mammalian: suitable
Cas Number25389-94-0
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