L3147-250G Display Image

LB Broth with agar (Miller), Powder microbial growth medium

Code: L3147-250G D2-231

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for s...


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Your Price
£63.20 250G

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

LB Broth with agar (Miller) has been used for the generation of single colony for Pseudomonas putida KT2440, plating E. coli and for the generation of pure colonies of C14 and MC8M6 of bacterial strains.

Features and Benefits

Miller’;s LB powder with agar provides: Easy scale-up using larger package sizes A budget-friendly alternative to pre-poured plates Standard formulation

General description

Miller’;s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Packaging

250 g in poly bottle

1 kg in poly bottle

Preparation Note

1. Suspend 40 g in 1L of distilled water.2. Heat to boiling while stirring to dissolve.3. Autoclave for 15 minutes at 121°C.4. Cool to 50°C prior to dispensing into sterile petri dishes.To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitution

Add 40g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).

application(s)food and beverages
compositionAgar, 15 g/L, NaCl, 10 g/L, Yeast Extract, 5 g/L, Tryptone, 10 g/L
formpowder
gradefor molecular biology
pH6.8-7.2(4% solution)
Quality Level200
sterilitynon-sterile
storage temp.room temp
suitabilitynonselective for Escherichia coli, nonselective for coliforms
Code
Description
Unit Size
List Price
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L3147-1KG
Unit:1KG
List Price: £191.00
Source:List Price
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