M0503-5X2MG Display Image
M0503-5X2MG Display Image

Mitomycin C from Streptomyces caespitosus, powder, contains NaCl as solubilizer

Code: M0503-5X2MG D2-231

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine gro...


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Your Price
£515.00 EACH

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biochem/physiol Actions

Mode of Action: This product is an alkylating agent that specifically targets the guanine nucleoside sequence 5′-CpG-3′. It inhibits DNA synthesis by covalently reacting with DNA, forming crosslinks between complementary strands of DNA. This interaction prevents separation of complementary DNA strands, inhibiting DNA replication.Antimicrobial Spectrum: Mitomycin C has strong antitumor activity, especially against Ehrlich ascites tumor cells, and strong bactericidal action against gram-positive and gram-negative bacteria.

Caution

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared - the precipitated solution has been proven toxic to cells.

General description

Chemical structure: aziridine

Preparation Note

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH﹤6, and is mostly likely to retain activity in solutions with a pH between 6-9.

 (1) 3.2, (2) 6.5
antibiotic activity spectrumGram-positive bacteria, Gram-negative bacteria
biological sourceStreptomyces caespitosus
colorgray, purple/blue
containsNaCl as solubilizer
formpowder
InChI keyNWIBSHFKIJFRCO-WUDYKRTCSA-N
InChI1S/C15H18N4O5/c1-5-9(16)12(21)8-6(4-24-14(17)22)15(23-2)13-7(18-13)3-19(15)10(8)11(5)20/h6-7,13,18H,3-4,16H2,1-2H3,(H2,17,22)/t6-,7+,13+,15-/m1/s1
mode of actionDNA synthesis | interferes
Quality Level200
SMILES string[H][C@]12CN3C4=C([C@@H](COC(N)=O)[C@@]3(OC)[C@@]1([H])N2)C(=O)C(N)=C(C)C4=O
solubilityH2O: 1 vial + 4 mL, clear to slightly hazy, blue to purple
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
M0503-10X2MG
Unit:EACH
List Price: £1,020.00
Source:List Price
ADD
M0503-2MG
Unit:2MG
List Price: £119.00
Source:List Price
ADD
M0503-50X2MG
Unit:EACH
List Price: £4,960.00
Source:List Price
ADD
Cas Number50-07-7
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