Application

M9 Minimal Salts, 5X is suitable for non-selective cultivation of Escherichia coli strains for cloning, DNA plasmid production and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Components

15 g/L KH₂PO₄64 g/L Na₂HPO₄ ⋅7H₂O2.5 g/L NaCl5.0 g/L NH₄Cl

Features and Benefits

M9 minimal media provides: Filter-sterilized, ready-to-use format Convenient package size Standard formulation

General description

M9 minimal media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.