Application

This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE343.

Immunogen

Puromycin-incorporated neosynthesized proteins at multiple molecular weights

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Protein G Purified

Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Quality

Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Specificity

This antibody detects Puromycin-incorporated neosynthesized proteins.

Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Refer to Cat. No. MABE343 for observed molecular weight information.