Application

Protamine sulfate has been used in the transduction oflentiviruses into human embryonic 293T cellsshRNA into mesenchymal stem cells/marrow stromal cells (MSC)lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34⁺ cells

Biochem/physiol Actions

Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.

General description

Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.

Packaging

10, 100 g in glass bottle