P4664-100TAB Display Image

o-Phenylenediamine dihydrochloride, tablet, 15 mg substrate per tablet

Code: P4664-100TAB D2-231

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as i...


read more

Your Price
£389.00 EACH

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 µl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

formtablet
InChI keyRIIWUGSYXOBDMC-UHFFFAOYSA-N
InChI1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
mp258 °C (dec.) (lit.)
Quality Level200
SMILES stringCl[H].Cl[H].Nc1ccccc1N
solubilitywater: 1 tablet/10 mL, clear, colorless
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
P4664-50TAB
Unit:EACH
List Price: £227.00
Source:List Price
ADD
Cas Number615-28-1
This product has met the following criteria:



Quantity
Est. Dispatch/Availability