P9187-50SET Display Image

SIGMAFAST OPD, tablet

Code: P9187-50SET D2-231

Application

SIGMAFAST OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of pero...


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£227.00 EACH

Application

SIGMAFAST OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay. It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.

Caution

This substrate is hygroscopic and light sensitive

Features and Benefits

SIGMAFAST OPD Tablets require no additional buffers or steps to prepare an active substrate solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Reconstitution

One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.

formtablet
Quality Level200
solubilitywater: 0.5 tablet/10 mL, clear, colorless
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
P9187-5SET
Unit:EACH
List Price: £45.30
Source:List Price
ADD
Hazard Class9
Un Number3316
Pack GroupIII
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