Application

Dialysis or buffer exchange of volumes between 10 mL and 20 mLPreparation of protein samples for MALDI-MS.Sample concentration.Large-scale protein dialysis such as antibodies and recombinant protein purification. Peptide dialysis for molecules as small as 10 amino acids. Virus-particle purification.Removal of contaminating micro molecules.Purification of tissue culture extracts.Removal of salts, surfactants, solvents and detergents.Complex formation studies (protein-protein, protein-DNA and protein-RNA)pH and buffer adjustment of sample solution, protein extract or cell extract. High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad. For your convenience the Pur-A-Lyzer kits come in four sizes: Mini: 10 - 250 µLMidi: 50 - 800 µLMaxi:0.1 - 3 mLMega: 3 - 20 mL

General description

The Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.

Legal Information

Pur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLC