T4444-100ML Display Image

3, 3,5 ,5-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution

Code: T4444-100ML D2-231

Application

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:to determine synthesized hyaluronan and H...


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Your Price
£99.90 100ML

Application

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectantto measure the levels of α-hemolysin toxinin imaging bound IgGs

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

General description

This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

formaqueous solution
InChI keyUAIUNKRWKOVEES-UHFFFAOYSA-N
InChI1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
Quality Level200
shipped inwet ice
SMILES stringCc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
storage temp.2-8°C
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